diff --git a/packages/core/src/ECS/Core/QuerySystem.ts b/packages/core/src/ECS/Core/QuerySystem.ts
index a852fad3..946d5a82 100644
--- a/packages/core/src/ECS/Core/QuerySystem.ts
+++ b/packages/core/src/ECS/Core/QuerySystem.ts
@@ -875,6 +875,7 @@ export class QuerySystem {
      *
      * 根据组件类型列表生成对应的位掩码。
      * 使用缓存避免重复计算。
+     * 注意:必须使用ComponentRegistry来确保与Entity.componentMask使用相同的bitIndex
      *
      * @param componentTypes 组件类型列表
      * @returns 生成的位掩码
@@ -891,10 +892,20 @@ export class QuerySystem {
             return cached;
         }
 
-        let mask = ComponentTypeManager.instance.getEntityBits(componentTypes);
+        // 使用ComponentRegistry而不是ComponentTypeManager,确保bitIndex一致
+        let mask = BitMask64Utils.clone(BitMask64Utils.ZERO);
+        for (const type of componentTypes) {
+            // 确保组件已注册
+            if (!ComponentRegistry.isRegistered(type)) {
+                ComponentRegistry.register(type);
+            }
+            const bitMask = ComponentRegistry.getBitMask(type);
+            BitMask64Utils.orInPlace(mask, bitMask);
+        }
+
         // 缓存结果
-        this.componentMaskCache.set(cacheKey, mask.getValue());
-        return mask.getValue();
+        this.componentMaskCache.set(cacheKey, mask);
+        return mask;
     }
 
     /**
@@ -1165,13 +1176,15 @@ export class QuerySystem {
      * 通知响应式查询实体已变化
      *
      * 使用混合策略:
-     * 1. 通知关心当前组件的查询
-     * 2. 通知当前包含该实体的查询(处理组件移除情况)
+     * 1. 首先通知关心实体当前组件的查询
+     * 2. 然后通知所有其他查询(包括那些可能因为组件移除而不再匹配的查询)
      *
      * @param entity 变化的实体
      */
     private notifyReactiveQueriesEntityChanged(entity: Entity): void {
-        if (this._reactiveQueries.size === 0) return;
+        if (this._reactiveQueries.size === 0) {
+            return;
+        }
 
         const notified = new Set<ReactiveQuery>();
 
@@ -1189,9 +1202,8 @@ export class QuerySystem {
         }
 
         for (const query of this._reactiveQueries.values()) {
-            if (!notified.has(query) && query.getEntities().includes(entity)) {
+            if (!notified.has(query)) {
                 query.notifyEntityChanged(entity);
-                notified.add(query);
             }
         }
     }
diff --git a/packages/core/tests/reactive-query-debug.test.ts b/packages/core/tests/reactive-query-debug.test.ts
new file mode 100644
index 00000000..f114e3f9
--- /dev/null
+++ b/packages/core/tests/reactive-query-debug.test.ts
@@ -0,0 +1,109 @@
+import { describe, it, expect, beforeEach } from '@jest/globals';
+import { Scene, Entity, Component, EntitySystem, Matcher, ECSComponent } from '../src';
+
+@ECSComponent('TestTransform')
+class TestTransform extends Component {
+    constructor(public x: number = 0, public y: number = 0) {
+        super();
+    }
+}
+
+@ECSComponent('TestRenderable')
+class TestRenderable extends Component {
+    constructor(public sprite: string = 'default') {
+        super();
+    }
+}
+
+class TestRenderSystem extends EntitySystem {
+    public entitiesFound: Entity[] = [];
+
+    constructor() {
+        super(Matcher.all(TestTransform, TestRenderable));
+    }
+
+    protected override process(entities: readonly Entity[]): void {
+        this.entitiesFound = Array.from(entities);
+        console.log(`TestRenderSystem.process: 找到 ${entities.length} 个实体`);
+    }
+
+    protected override onAdded(entity: Entity): void {
+        console.log(`TestRenderSystem.onAdded: 实体 ${entity.name}(${entity.id}) 被添加`);
+    }
+}
+
+describe('响应式查询调试', () => {
+    let scene: Scene;
+    let system: TestRenderSystem;
+
+    beforeEach(() => {
+        scene = new Scene();
+        system = new TestRenderSystem();
+        scene.addEntityProcessor(system);
+        scene.begin();
+    });
+
+    it('应该在实体添加组件后能被System发现', () => {
+        console.log('\n=== 测试开始 ===');
+
+        // 1. 创建实体（此时没有组件）
+        console.log('\n步骤1: 创建实体');
+        const entity = scene.createEntity('TestEntity');
+        console.log(`实体已创建: ${entity.name}(${entity.id})`);
+        console.log(`QuerySystem中的实体数量: ${scene.querySystem.getAllEntities().length}`);
+
+        // 2. 添加组件
+        console.log('\n步骤2: 添加 TestTransform 组件');
+        entity.addComponent(new TestTransform(100, 200));
+        console.log(`实体组件数量: ${entity.components.length}`);
+
+        console.log('\n步骤3: 添加 TestRenderable 组件');
+        entity.addComponent(new TestRenderable('test-sprite'));
+        console.log(`实体组件数量: ${entity.components.length}`);
+
+        // 3. 触发系统更新
+        console.log('\n步骤4: 更新Scene');
+        scene.update();
+
+        // 4. 检查System是否找到了实体
+        console.log(`\nSystem找到的实体数量: ${system.entitiesFound.length}`);
+        if (system.entitiesFound.length > 0) {
+            console.log(`找到的实体: ${system.entitiesFound.map(e => `${e.name}(${e.id})`).join(', ')}`);
+        }
+
+        // 5. 直接查询QuerySystem
+        console.log('\n步骤5: 直接查询QuerySystem');
+        const queryResult = scene.querySystem.queryAll(TestTransform, TestRenderable);
+        console.log(`QuerySystem.queryAll 返回: ${queryResult.entities.length} 个实体`);
+
+        console.log('\n=== 测试结束 ===\n');
+
+        expect(system.entitiesFound.length).toBe(1);
+        expect(system.entitiesFound[0]).toBe(entity);
+        expect(queryResult.entities.length).toBe(1);
+        expect(queryResult.entities[0]).toBe(entity);
+    });
+
+    it('应该测试响应式查询的内部状态', () => {
+        console.log('\n=== 响应式查询内部状态测试 ===');
+
+        // 创建实体并添加组件
+        const entity = scene.createEntity('TestEntity');
+        entity.addComponent(new TestTransform(100, 200));
+        entity.addComponent(new TestRenderable('test-sprite'));
+
+        // 获取QuerySystem的内部状态
+        const querySystem = scene.querySystem as any;
+        console.log(`\n响应式查询数量: ${querySystem._reactiveQueries.size}`);
+        console.log(`组件索引数量: ${querySystem._reactiveQueriesByComponent.size}`);
+
+        // 检查响应式查询
+        for (const [key, query] of querySystem._reactiveQueries) {
+            console.log(`\n查询: ${key}`);
+            console.log(`  实体数量: ${(query as any)._entities.length}`);
+            console.log(`  实体ID集合: ${Array.from((query as any)._entityIdSet).join(', ')}`);
+        }
+
+        console.log('\n=== 测试结束 ===\n');
+    });
+});
diff --git a/packages/core/tests/reactive-query-timing.test.ts b/packages/core/tests/reactive-query-timing.test.ts
new file mode 100644
index 00000000..c6aa760c
--- /dev/null
+++ b/packages/core/tests/reactive-query-timing.test.ts
@@ -0,0 +1,89 @@
+import { describe, it, expect } from '@jest/globals';
+import { Scene, Entity, Component, EntitySystem, Matcher, ECSComponent } from '../src';
+
+@ECSComponent('TestTransform')
+class TestTransform extends Component {
+    constructor(public x: number = 0, public y: number = 0) {
+        super();
+    }
+}
+
+@ECSComponent('TestRenderable')
+class TestRenderable extends Component {
+    constructor(public sprite: string = 'default') {
+        super();
+    }
+}
+
+class TestRenderSystem extends EntitySystem {
+    public entitiesFound: Entity[] = [];
+
+    constructor() {
+        super(Matcher.all(TestTransform, TestRenderable));
+    }
+
+    protected override process(entities: readonly Entity[]): void {
+        this.entitiesFound = Array.from(entities);
+        console.log(`TestRenderSystem.process: 找到 ${entities.length} 个实体`);
+    }
+
+    protected override onAdded(entity: Entity): void {
+        console.log(`TestRenderSystem.onAdded: 实体 ${entity.name}(${entity.id}) 被添加`);
+    }
+}
+
+class TestGameScene extends Scene {
+    private renderSystem: TestRenderSystem | null = null;
+
+    public override initialize(): void {
+        super.initialize();
+
+        console.log('\n=== Scene.initialize() 开始 ===');
+
+        // 1. 先添加System（这是GameScene的做法）
+        console.log('步骤1: 添加RenderSystem');
+        this.renderSystem = new TestRenderSystem();
+        this.addEntityProcessor(this.renderSystem);
+
+        // 2. 然后创建实体（这是GameScene的做法）
+        console.log('\n步骤2: 创建实体');
+        const entity = this.createEntity('Player');
+        console.log(`实体已创建: ${entity.name}(${entity.id})`);
+
+        console.log('\n步骤3: 添加组件');
+        entity.addComponent(new TestTransform(100, 200));
+        entity.addComponent(new TestRenderable('player-sprite'));
+        console.log(`实体组件数量: ${entity.components.length}`);
+
+        console.log('=== Scene.initialize() 结束 ===\n');
+    }
+
+    public getRenderSystem(): TestRenderSystem | null {
+        return this.renderSystem;
+    }
+}
+
+describe('响应式查询时序测试（模拟GameScene）', () => {
+    it('应该在Scene.initialize()中先添加System再创建实体时正常工作', () => {
+        console.log('\n\n========== 测试开始 ==========');
+
+        const scene = new TestGameScene();
+
+        console.log('\n调用scene.initialize()');
+        scene.initialize();
+
+        console.log('\n调用Scene.begin()');
+        scene.begin();
+
+        console.log('\n第一次Scene.update()');
+        scene.update();
+
+        const renderSystem = scene.getRenderSystem();
+        expect(renderSystem).not.toBeNull();
+
+        console.log(`\nRenderSystem找到的实体数量: ${renderSystem!.entitiesFound.length}`);
+        expect(renderSystem!.entitiesFound.length).toBe(1);
+
+        console.log('========== 测试结束 ==========\n\n');
+    });
+});